![]() ![]() Ligand coupling method: aldehyde coupling via NH 2 residues of the ligandīinding capacity: 25–35 g IgG/L resin depending on flow rate, column height, and contact timeĮlution conditions: 20 mM citric acid or acetic acid, pH 3–4, or 20 mM sodium acetate, 1. Ligand: CaptureSelect™ FcXL affinity ligand Matrix: agarose-based, aldehyde-activated The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free). Capto S ImpAct is based on a high-flow agarose base matrix with good pressure/flow properties and an average bead size of 50 m, which combined with an optimized porosity gives high resolution. The small bead size of Capto S ImpAct allows for. Pressure contribution from system, tubings and column is excluded. Strong cation exchanger for high-productivity capture and intermediate purification when high volume throughput is essential. Running conditions: AxiChrom 300 (30 cm i.d.), 20 cm bed height with Packing factor 1.20 in 0.15 M NaCl at 20C. Capto S is a strong cation exchanger for fast, efficient, and cost-effective capture and intermediate protein purification from large feed volumes. Human specific, no binding to bovine antibodies, for exampleĬaptureSelect™ FcXL Affinity Matrix purifies IgGs and Fc fusion proteins directly from complex source materials in a single step with high purity and yield.ĬaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. Check chemical resistance with the connector supplier. Fig 2.Example of pressure flow curves for Capto S ImpAct in open bed (left) and packed bed (right), respectively.Mild elution, making it suitable for Fc fusion proteins.It recognizes the CH3 domain of all human IgG subclasses (IgG1, 2, 3, and 4).įeatures of this affinity matrix include: ![]() CaptureSelect™ FcXL Affinity Matrix has been specifically designed for the purification of recombinant human IgG, Fc fusion proteins, and plasma derived IgG. ![]()
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